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銆€銆€鏃ュ墠錛岀敱寮楅浄寰峰搱閽︽．鐧岀棁鐮旂┒涓績(Fred Hutchinson Cancer Research Center)棰嗗鐨勪竴涓浗闄呯爺絀跺皬緇勮瘉瀹炰簡澶ц妯°€佹爣鍑嗗寲铔嬬櫧璐ㄦ嫻嬬殑鍙鎬э紝榪欐槸楠岃瘉鐤劇梾鐢熺墿鏍囧織鐗╁拰鑽墿闈剁偣鐨勫繀瑕佹潯浠躲€傝繖欏瑰垔鐧誨湪銆婅嚜鐒?鏂規(guī)硶銆?Nature Methods)鏉傚織涓婄殑鏈€鏂拌鏂囪〃鏄庯紝縐戝瀹朵滑寮€鍙戠殑涓€縐嶉澏鍚戞€ц泲鐧借川媯€(gè)嫻嬫柟娉曞叿鏈夌郴緇熷湴銆佸彲闈犲湴媯€(gè)嫻嬩漢綾昏泲鐧借川緇勭殑娼滃姏銆?/span>
銆€銆€璁烘枃涓昏浣滆€呫€佺檶鐥囪泲鐧借川緇勫涓撳 Amanda Paulovich 鍗氬＋鍜屽悓浜嬩滑寮€鍙戠殑榪欓」鎶€鏈紝鍙悓鏃跺噯紜湴媯€(gè)嫻嬭澶氫笉鍚屾牱鏈腑鎴愮櫨涓婂崈縐嶈泲鐧借川鐨勪赴搴︺€傛潵鑷タ闆呭浘銆佹嘗澹】鍜岄煩鍥界瓑鍏朵粬鍦板尯鐨勫疄楠屽閲嶇幇浜嗕漢綾諱鉤鑵虹檶緇嗚優(yōu)涓?319 縐嶈泲鐧借川鐨勬嫻嬬粨鏋滐紝璇佸疄榪欑鏂規(guī)硶鍙法瓚婂疄楠屽鍜屽浗鐣屽疄鐜版爣鍑嗗寲銆?/span>
銆€銆€Paulovich 琛ㄧず錛氣€滆繖縐嶆柟娉曟湁娼滃姏褰誨簳鏀瑰彉鎴戜滑媯€(gè)嫻嬩漢綾昏泲鐧借川鐨勬柟寮忋€傚埄鐢ㄥ叏鐞冭祫婧愬鎵€鏈変漢綾昏泲鐧借川榪涜鏍囧噯鍖栧畾閲忚绔嬩竴浜涙柊鏍囧噯錛屾棤鐤戝皢鑳芥彁楂樹復(fù)搴婄爺絀剁殑鍙噸鐜版€э紝鍏跺皢瀵硅漿鍖栨柊鍨嬫不鐤楀拰璇婃柇甯︽潵宸ㄥぇ鐨勫獎(jiǎng)鍝嶃€傗€?/span>
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銆€銆€姣忎釜鏈夊墠鏅殑鐢熺墿鏍囪鐗╅兘蹇呴』鍦ㄤ復(fù)搴婅瘯楠屼腑寮€灞曡繘涓€姝ョ殑鐮旂┒錛岃繖灝辮姹傜爺絀朵漢鍛樿兘澶熸嫻嬫暟鐧懼埌鏁板崈涓?zhèn)ｈ€呮牱鏈腑姣忎釜鍊欓€夋爣蹇楃墿鐨勪赴搴︺€傜敱浜庡皢浠諱綍涓€縐嶅€欓€夋爣璁扮墿杞寲鑷充復(fù)搴婂簲鐢ㄧ殑鏈虹巼閮芥瀬鍏剁殑浣庯紝閴村埆涓€縐嶅叿鏈変復(fù)搴婁環(huán)鍊肩殑鐢熺墿鏍囪鐗╁繀欏誨澶ч噺鐨勮泲鐧借川榪涜嫻嬭瘯銆?/span>
銆€銆€Paulovich 琛ㄧず錛氣€滅幇鍦紝浣犺繕涓嶈兘瀵瑰ぇ澶氭暟鐨勪漢綾昏泲鐧借川榪涜澶ц妯℃嫻嬨€傚湪鎴戜滑瀹屾垚浜虹被鍩哄洜緇勬祴搴忥紝鑾峰緱DNA鍒嗗瓙鍏ㄧ洰褰?0澶氬勾涔嬪悗錛屼粛鐒朵笉鑳藉閲囩敤涓€縐嶆爣鍑嗗寲瀹氶噺鏂規(guī)硶鍦ㄥ悇縐嶉€氶噺妯″紡涓嬪浜虹被铔嬬櫧璐ㄧ粍寮€灞曠爺絀躲€傗€?/span>
銆€銆€涓轟簡瑙ｅ喅榪欎竴闂錛孭aulovich 鍜屽悓浜嬩滑鍒╃敤浜嗕竴縐嶇О浣滀負(fù)澶氬弽搴旀嫻嬭川璋辨硶(MRM-MS)鐨勬晱鎰熸€ч澏鍚戣泲鐧借川媯€(gè)嫻嬫妧鏈€傝繖縐嶈川璋辨硶騫墮潪鏄叏鏂扮殑鎶€鏈紝澶氬勾鏉ュ叏鐞冪殑涓村簥瀹為獙瀹ゅ埄鐢ㄥ畠鏉ユ祴閲忚嵂鐗╀唬璋駭鐗╁拰涓庡厛澶╂€т唬璋㈢己闄鋒湁鍏崇殑涓€浜涘皬鍒嗗瓙銆傛渶榪戯紝Paulovich鍜屽叾浠栫爺絀朵漢鍛樺紑濮嬪埄鐢ㄥ畠鏉ユ嫻嬩漢綾昏泲鐧借川銆?/span>
銆€銆€閲囩敤鐮旂┒浜哄憳寮€鍙戠殑榪欑鏂規(guī)硶錛屾瘡澶╂瘡鍙頒華鍣ㄨ兘澶熷鏈€灝?20 涓復(fù)搴婃牱鏈腑鐨?170 縐嶈泲鐧借川榪涜楂樺害鐗瑰紓鎬у湴銆佺簿紜湴銆佸璺畾閲忓垎鏋?浠諱綍鍏朵粬鐨勭幇鏈夋妧鏈兘娌℃湁榪欑鑳藉姏銆?/span>
銆€銆€鐢變簬璐ㄨ氨鎶€鏈槸閽堝鎬х殑錛岃繖鎰忓懗鐫€鐮旂┒浜哄憳鑳藉璋冩暣璁懼瀵繪壘鐧岀粏鑳?yōu)鎴栧叾浠栨牱鍝伨c誨瀷涓壒孌婄殑铔嬬櫧璐ㄤ簹緹わ紝鐩告瘮浜庨潪閽堝鎬х瓥鐣ワ紝瀹冨彲浠ュ湪鏇翠綆鐨勬按騫充笂媯€(gè)嫻嬪井閲忚娑叉牱鏈垨媧繪鏍囨湰涓洰鐨勮泲鐧借川鐨勫瓨鍦ㄣ€?/span>
銆€銆€鐮旂┒鐨勪富瑕佷綔鑰呫€丳aulovich 瀹為獙瀹ゅ垎鏋愬寲瀛﹀ Jacob Kennedy 璇達(dá)細(xì)鈥滄垜浠殑鐩爣鏄敤榪欎竴鎶€鏈潵鍙栦唬褰撳墠閲囩敤鐨勪竴浜涢潪甯歌€佹棫鐨勬妧鏈€傗€?/span>
銆€銆€褰撳墠錛岀爺絀朵漢鍛橀€氬父鏄噰鐢?Western blotting銆丒LISA 鎴栨槸鍏嶇柅緇勫寲(IHC)鎶€鏈潵媯€(gè)嫻嬩復(fù)搴婃牱鏈腑鐨勮泲鐧借川姘村鉤銆傝繖浜涙柟娉曞線寰€鏃犳硶鍦ㄥ疄楠屽涔嬮棿閲嶇幇緇撴灉錛屼粠鑰屽緢闅鵑獙璇侀€傜敤浜庝復(fù)搴婄殑鍊欓€夌敓鐗╂爣璁扮墿錛屽畠浠笉閫傜敤浜庝竴嬈℃嫻嬪ぇ閲忕殑铔嬬櫧璐ㄥ拰鏍鋒湰銆?/span>
銆€銆€Paulovich 鍜屽悓浜嬩滑閫氳繃鍒嗘瀽涔寵吅鐧岀粏鑳?yōu)鐢熸垚鐨?300 澶氱宸茬煡铔嬬櫧璐ㄩ獙璇佷簡浠栦滑鐨勬妧鏈?鐮旂┒緇撴灉琛ㄦ槑錛孧RM-MS 鍙互閲嶇幇鍙?qiáng)鎵╁睍浠ュ線閲囩敤鍏朵粬鎶€鏈繘琛屼鉤鑵虹檶鐮旂┒鎵€鐢熸垚鐨勮瀵熺粨鏋溿€?/span>
銆€銆€璇ョ爺絀惰瘉瀹炰簡錛孧RM-MS 鑳藉浠ヤ竴縐嶆爣鍑嗗寲鏂瑰紡涓€嬈℃嫻嬭澶氱殑铔嬬櫧璐紝涓哄紑灞曞浗闄呮€х殑銆佹湁緇勭粐鐨勭爺絀跺伐浣滃畾閲忎漢綾昏泲鐧借川涓殑姣忕铔嬬櫧濂犲畾浜嗗熀紜€銆?/span>
銆€銆€璁烘枃涓昏浣滆€呫€佺檶鐥囪泲鐧借川緇勫涓撳 Amanda Paulovich 鍗氬＋鍜屽悓浜嬩滑寮€鍙戠殑榪欓」鎶€鏈紝鍙悓鏃跺噯紜湴媯€(gè)嫻嬭澶氫笉鍚屾牱鏈腑鎴愮櫨涓婂崈縐嶈泲鐧借川鐨勪赴搴︺€傛潵鑷タ闆呭浘銆佹嘗澹】鍜岄煩鍥界瓑鍏朵粬鍦板尯鐨勫疄楠屽閲嶇幇浜嗕漢綾諱鉤鑵虹檶緇嗚優(yōu)涓?319 縐嶈泲鐧借川鐨勬嫻嬬粨鏋滐紝璇佸疄榪欑鏂規(guī)硶鍙法瓚婂疄楠屽鍜屽浗鐣屽疄鐜版爣鍑嗗寲銆?/span>
銆€銆€Paulovich 琛ㄧず錛氣€滆繖縐嶆柟娉曟湁娼滃姏褰誨簳鏀瑰彉鎴戜滑媯€(gè)嫻嬩漢綾昏泲鐧借川鐨勬柟寮忋€傚埄鐢ㄥ叏鐞冭祫婧愬鎵€鏈変漢綾昏泲鐧借川榪涜鏍囧噯鍖栧畾閲忚绔嬩竴浜涙柊鏍囧噯錛屾棤鐤戝皢鑳芥彁楂樹復(fù)搴婄爺絀剁殑鍙噸鐜版€э紝鍏跺皢瀵硅漿鍖栨柊鍨嬫不鐤楀拰璇婃柇甯︽潵宸ㄥぇ鐨勫獎(jiǎng)鍝嶃€傗€?/span>
銆€銆€浣滀負(fù)鎵€鏈夌敓鐗╁姛鑳界殑鎵ц鍒嗗瓙鏈哄櫒錛岃泲鐧借川鎺屾帶鐫€鏃╂湡鐤劇梾鍜岀柧鐥呰繘紼嬬殑淇″彿浼犲銆傛帰姹傜檶鐥囩敓鐗╂爣璁扮墿鈥斺€旂粏鑳?yōu)涓殑铔嬬櫧璐ㄦ寚绾规湁鍙兘淇冧娇寮€鍙戝嚭涓€浜涙祴璇曟柟娉曪紝鏇存棭鏈熷湴媯€(gè)嫻嬬柧鐥咃紝鏃╁湪鐧岀棁褰㈡垚涔嬪墠閴村埆鍑轟釜浣撶殑鐗規(guī)畩椋庨櫓錛屼互鍙?qiáng)鏇村ソ鍦版寚瀵兼?zhèn)ｈ€呯殑娌葷枟銆傜劧鑰屾病鏈夋爣鍑嗗寲鍜屽彲閲嶇幇鐨勬柟娉曟潵媯€(gè)嫻嬪畠浠殑姘村鉤錛岄獙璇佹柊鍙戠幇鐨勫€欓€夌敓鐗╂爣璁扮墿鏄竴浠朵笉鍙兘鐨勪簨鎯呫€?/span>
銆€銆€姣忎釜鏈夊墠鏅殑鐢熺墿鏍囪鐗╅兘蹇呴』鍦ㄤ復(fù)搴婅瘯楠屼腑寮€灞曡繘涓€姝ョ殑鐮旂┒錛岃繖灝辮姹傜爺絀朵漢鍛樿兘澶熸嫻嬫暟鐧懼埌鏁板崈涓?zhèn)ｈ€呮牱鏈腑姣忎釜鍊欓€夋爣蹇楃墿鐨勪赴搴︺€傜敱浜庡皢浠諱綍涓€縐嶅€欓€夋爣璁扮墿杞寲鑷充復(fù)搴婂簲鐢ㄧ殑鏈虹巼閮芥瀬鍏剁殑浣庯紝閴村埆涓€縐嶅叿鏈変復(fù)搴婁環(huán)鍊肩殑鐢熺墿鏍囪鐗╁繀欏誨澶ч噺鐨勮泲鐧借川榪涜嫻嬭瘯銆?/span>
銆€銆€Paulovich 琛ㄧず錛氣€滅幇鍦紝浣犺繕涓嶈兘瀵瑰ぇ澶氭暟鐨勪漢綾昏泲鐧借川榪涜澶ц妯℃嫻嬨€傚湪鎴戜滑瀹屾垚浜虹被鍩哄洜緇勬祴搴忥紝鑾峰緱DNA鍒嗗瓙鍏ㄧ洰褰?0澶氬勾涔嬪悗錛屼粛鐒朵笉鑳藉閲囩敤涓€縐嶆爣鍑嗗寲瀹氶噺鏂規(guī)硶鍦ㄥ悇縐嶉€氶噺妯″紡涓嬪浜虹被铔嬬櫧璐ㄧ粍寮€灞曠爺絀躲€傗€?/span>
銆€銆€涓轟簡瑙ｅ喅榪欎竴闂錛孭aulovich 鍜屽悓浜嬩滑鍒╃敤浜嗕竴縐嶇О浣滀負(fù)澶氬弽搴旀嫻嬭川璋辨硶(MRM-MS)鐨勬晱鎰熸€ч澏鍚戣泲鐧借川媯€(gè)嫻嬫妧鏈€傝繖縐嶈川璋辨硶騫墮潪鏄叏鏂扮殑鎶€鏈紝澶氬勾鏉ュ叏鐞冪殑涓村簥瀹為獙瀹ゅ埄鐢ㄥ畠鏉ユ祴閲忚嵂鐗╀唬璋駭鐗╁拰涓庡厛澶╂€т唬璋㈢己闄鋒湁鍏崇殑涓€浜涘皬鍒嗗瓙銆傛渶榪戯紝Paulovich鍜屽叾浠栫爺絀朵漢鍛樺紑濮嬪埄鐢ㄥ畠鏉ユ嫻嬩漢綾昏泲鐧借川銆?/span>
銆€銆€閲囩敤鐮旂┒浜哄憳寮€鍙戠殑榪欑鏂規(guī)硶錛屾瘡澶╂瘡鍙頒華鍣ㄨ兘澶熷鏈€灝?20 涓復(fù)搴婃牱鏈腑鐨?170 縐嶈泲鐧借川榪涜楂樺害鐗瑰紓鎬у湴銆佺簿紜湴銆佸璺畾閲忓垎鏋?浠諱綍鍏朵粬鐨勭幇鏈夋妧鏈兘娌℃湁榪欑鑳藉姏銆?/span>
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