瓚呴珮鍒嗚鯨鐜?/span>鑽у厜鏄懼井闀滄槸榪?/span>騫?/span>鏉?/span>鍏磋搗鐨?/span>鏂版妧鏈?/span>錛?/span>瀹冨彲浠ヨ秴瓚婅繙鍦哄厜瀛︽樉寰暅鐨勫垎杈ㄧ巼鏋侀檺錛屽嵆闃胯礉鏋侀檺錛?00綰崇背宸﹀彸錛夛紝鐩存帴媯嫻嬪埌鍑犲崄綰崇背鐨勭簿緇嗙粨鏋勩備笌鑳借揪鍒扮浉鍚屾垨鏇撮珮鍒嗚鯨鐜囩殑X鍏夋樉寰暅銆佸悇綾葷數瀛愭樉寰暅鍙婂師瀛愬姏鏄懼井闀滅浉姣旓紝瓚呴珮鍒嗚鯨鑽у厜鎴愬儚鐨勪紭鍔挎槸鍦ㄥ父娓╁父鍘嬪拰鍩烘湰涓嶆崯浼ょ敓鐗╂牱鏈椿鎬х殑鏉′歡涓嬶紝鑾峰緱鍏剁撼綾沖昂搴︾殑鍥懼儚淇℃伅銆?/span>
鍏跺熀鏈師鐞嗘槸鐢?/span>鍏夊垏鎹?/span> (photoswitchable) 鎴?/span>鍏夋椿鍖?photoactivable) 鑽у厜鎺㈤拡鏍囪鏍鋒湰錛岄氳繃绱媧誨寲鍏夋縺媧誨皯閲忚崸鍏夋帰閽堬紝鍒╃敤璇誨嚭鍏夎繘琛?/span>鍗曞垎瀛愭垚鍍?/span>錛屽啀瀵瑰叾榪涜(Point spread function)-鐐瑰垎甯冨嚱鏁版嫙鍚堬紝鍦ㄧ┖闂翠笂綺懼害瀹氫綅銆傚洜涓哄彧鏈夊皯閲忚崸鍏夋帰閽堣嬋媧伙紝鎺㈤拡闂村鉤鍧囪窛紱誨ぇ浜庨樋璐濇瀬闄愮殑鎯呭喌涓嬶紝鎵嶈兘鍑嗙‘鍒嗚鯨姣忎竴涓崸鍏夊垎瀛愬茍綺劇‘瀹氫綅銆傚皢澶ч噺鍗曞垎瀛愬畾浣嶄俊鎭彔鍔狅紝鐢辨鑾峰緱瓚呰秺闃胯礉鏋侀檺鐨?/span>瓚?/span>楂樺垎杈ㄧ巼鐨勫厜瀛?/span>鍥懼儚錛岀悊鎯蟲儏鍐典笅鍙互杈懼埌鎴栧ソ浜?0綰崇背鍒嗚鯨鐜?/span>錛岀敱姝ょО涔嬩負闅忔満鍏夊閲嶅緩鏄懼井闀?(Stochastic Optical Reconstruction Microscopy, STORM) 鎴栧厜媧誨寲瀹氫綅鏄懼井闀?Photo-Activated Localization Microscopy, PALM)銆?/span>
鍦ㄧ鎶閮ㄣ佸浗瀹惰嚜鐒剁瀛﹀熀閲戝銆佷腑鍥界瀛﹂櫌鍜屽寲瀛︽墍鐨勬敮鎸佷笅錛岃兌浣撱佺晫闈笌鍖栧鐑姏瀛﹂櫌閲嶇偣瀹為獙瀹ょ殑鐮旂┒浜哄憳鍦ㄨ秴楂樺垎杈ㄥ浘鍍忛噰闆?/span>鍜?/span>鏁版嵁鍒嗘瀽鏂歸潰鍙戝睍浜嗗疄鏃跺崟鍒嗗瓙瀹氫綅鐨勭▼搴忓寘SNSMIL錛岃紼嬪簭鍖呭彲騫挎硾搴旂敤浜庨珮鑳屾櫙鎴愬儚鐨勬暟鎹垎鏋愶紙Scientific Reports 2015, 5,11073錛夈傚悓鏃訛紝鐢ㄦ鏂規硶鑾峰緱浜嗚緇嗚優鍐呭悶鍚?/span>鐨勫婧?/span>鑴傝川浣撳湪緇嗚優楠ㄦ灦涓婄殑鍒嗗竷鍜岃繍鍔ㄨ建榪?/span>錛屼負鐮旂┒鑴傝川浣撴垨澶?/span>婧?/span>緇勮浣?/span>涓庣粏鑳炰綔鐢?/span>鎻愪緵浜嗛噸瑕佺殑媯嫻?/span>鎵嬫錛?/span>Scientific Reports 2015, 5, 16559錛夈?/span>
鍦ㄥ墠鏈熺爺絀朵腑錛岀爺絀朵漢鍛樺埄鐢ㄥ垎瀛愮粍瑁呮妧鏈瀯寤轟簡澶氬瓟紕抽吀閽?綰崇背綺掑瓙澶嶅悎鑳朵綋棰楃矑錛?/span>Adv. Mater. 2012, 24, 2663鈥?667錛夛紝騫惰幏寰椾簡鍔熻兘鍖栫殑鐜鍝嶅簲鎬у井鐞冿紙Adv. Funct. Mater. 2012, 22, 2673鈥?681錛夈傛渶榪戜粬浠嬈″埄鐢?(d)STORM瑙傛祴鍒扮敓鐗╃熆鍖栬繃紼嬩腑鍙備笌緇撴櫠鐨勮泲鐧借川鍒嗗竷淇℃伅錛屼負鐮旂┒铔嬬櫧璐ㄨ瀵肩敓鐗╃熆鍖栫殑鏈虹悊鎻愪緵浜嗛噸瑕佺殑瀹為獙鎵嬫(Angew. Chem. Int. Ed. 2016, 55, 908-911錛?/span>銆?/span>
Gelatin鍦ㄦ柟瑙ｇ煶涓垎甯冪殑瓚呴珮鍒嗚鯨鑽у厜鎴愬儚
