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Regenerated silk fibroin (RSF) fibres were obtained by extruding a concentrated aqueous silk fibroin solution into an ammonium sulphate coagulation bath. A custom-made simplified industrial-type wet-spinning device with continuous mechanical post-draw was used. The effect of dope concentration, coagulation bath, extrusion rate and post-draw treatment on the morphology of RSF fibre was examined. The results showed that although RSF fibre could be formed with dope concentration between 13% and 19% (w/w), the ones spun from 15% RSF solution showed the most regular morphology being dense and homogenous in cross-section with a smooth surface and a uniform cylindrical shape. Though it had a little effect on morphology, post-draw treatment especially under steam significantly improved the mechanical properties of the RSF fibres.