私密直播全婐app免费大渔直播,国产av成人无码免费视频,男女同房做爰全过程高潮,国产精品自产拍在线观看

相關(guān)鏈接
聯(lián)系方式
  • 通信地址:武漢大學(xué)化學(xué)與分子科學(xué)學(xué)院
  • 郵編:430072
  • 電話:027-68755317
  • 傳真:027-68755317
  • Email:swhuang@whu.edu.cn
當(dāng)前位置:> 首頁 > 論文著作 > 正文
ACTA POLYMERICA SINICA 2011: POLYASPARTAMIDES GRAFTED WITH 1-(3-AMINOPROPYL)IMIDAZOLE AS LESS TOXIC AND HIGHLY EFFICIENT GENE CARRIERS
作者:Xue Ya-Nan, Zhang Min, Liu Lei, Li Wen-Xin, Huang Shi-Wen
關(guān)鍵字:Gene delivery; Polycation; Polyaspartamide; Imidazole; Less toxic
論文來源:期刊
具體來源:ACTA POLYMERICA SINICA 2011, (8) 874-882
發(fā)表時間:2011年

Polyaspartamides containing pendant imidazolyl propyl groups and amino ethyl groups were synthesized via ring-opening of polysuccinimide (PSI) reacting with N-Boc protected ethylenediamine and 1-3-aminopropyl) imidazole. Polyaspartamides (P1 similar to P5) with different grafting ratios were characterized by FTIR and (1)H-NMR spectra, which were well in accordance with the expected chemical structures. The cytotoxicity of polymers was evaluated by MTT assay. Compared to the commercial branched polyethyleneimine (PEI) (25 kDa) with an IC50 of 15 mu g/mL, P1 similar to P5 showed no significant cytotoxicity against both 293T cells and COS-7 cells. More than 90% cells still retained their metabolic ability even at the polymer concentration up to 0.8 mg/mL. The introduction of imidazolyl group into polymer side chains make them possess high buffer capacity between pH 5 and pH 7, which was 2 similar to 5 times higher than that of PEI. Gel electrophoresis assay and dynamic light scattering measurements were performed to characterize the polycation/DNA complexes. At an N/P ratio of above 5, all of the five polyaspartamide-based polycations can condense plasmid DNA to form nanosized (100 nm) polyelectrolyte complexes with positive surface charge (+20 mV). The transfection assay demonstrated that the transfection efficiencies of foreign DNA in 293T cells and COS-7 cells increased with increasing the contents of imidazolyl graft of polyaspartamides. P4 with a grafting ratio of 1/3.7 (amino ethylene group to imidazolyl propyl group) showed the highest ability to deliver pGL-3 and pEGFP-C1 into both 293T cells and COS-7 cells. At the optimal N/P ratios, the gene expression efficiencies mediated by P4 were comparable to or even 2 similar to 3 times higher than that of PEI. The results suggest that imidazole-containing polyaspartamides are a very promising class of novel polycations for highly efficient and less toxic gene delivery. The structure-gene delivery ability relationship of imidazole-grafted polyaspartamides will provide valuable insight into the development of novel nonviral gene carrier.

主站蜘蛛池模板: 南陵县| 克山县| 开鲁县| 都昌县| 肥西县| 富宁县| 竹山县| 铜川市| 北辰区| 林西县| 临海市| 宜黄县| 乐至县| 连州市| 韩城市| 德钦县| 苗栗市| 天镇县| 海兴县| 娱乐| 新营市| 庄浪县| 普安县| 正安县| 安陆市| 寻乌县| 乳源| 金昌市| 澄迈县| 新干县| 滦平县| 三原县| 增城市| 信宜市| 临猗县| 宕昌县| 梅州市| 喀喇沁旗| 陕西省| 星子县| 阿坝|