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【ACS Applied Materials & Interfaces】Fabrication of a detection platform with boronic acid containing zwitterionic polymer brush
作者:Lingjie Song, Shifang Luan*, Jiao Ma, Jingchuan Liu, Xiaodong Xu, Jinghua Yin*, et al.,
關鍵字:poly(styrene-b-isobutylene-b-styrene) (SIBS), deoxyribonuclease (DNase), ribonuclease (RNase), antibacterial, cell adhesion
論文來源:期刊
具體來源:ACS Applied Materials & Interfaces, 2013, 5, 13207-13215
發表時間:2013年

Abstract:

Development of technologies for biomedical detection platform is critical to meet the global challenges of various disease diagnoses, especially for point-of-use applications. Because of its natural simplicity, effectiveness, and easy repeatability, random covalent-binding technique is widely adopted in antibody immobilization. However, its antigen-binding capacity is relatively low when compared to site-specific immobilization of antibody. Herein, we report that a detection platform modified with boronic acid (BA)-containing sulfobetaine-based polymer brush. Mainly because of the advantage of oriented immobilization of antibody endowed with BA-containing three-dimensional polymer brush architecture, the platform had a high antigen-binding capacity. Notably, nonspecific protein adsorption was also suppressed by the zwitterionic pendants, thus greatly enhanced signal-to-noise (S/N) values for antigen recognition. Furthermore, antibodies captured by BA pendants could be released in dissociation media. This new platform is promising for potential applications in immunoassays.


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