項目類別：國家自然科學基金
項目編號：21574050
項目規模：面上項目
參與人員：楊光（主持）、石志軍、黃芳、李瑩、黃亞佳、劉莉、李思祥、曾迪
起止日期：2016.01-2019.12
生物體產生的內源信號在發育、再生以及自我修復過程起著舉足輕重的作用。本項目
采用已建立的生物制造過程控制的方法，在生物合成過程中經分子模板和微流控調控葡糖
醋桿菌的定向運動，此外通過構建趨光型葡糖木醋桿菌工程菌株，控制纖維素纖維的組裝
與排列，光控合成具有特定規則圖案的三維的纖維素材料，為組織細胞提供多層次高度精
細結構的組織工程支架，誘導其與支架的定向黏附和聚集。進一步通過電活性修飾，模擬
生物體的內源生物電信號，引導和調控成纖維細胞、神經元細胞、內皮細胞的分布、遷移
、增殖、分化等。本項目針對“調控內源信號實現無損定向誘導皮膚組織的修復與再生”
的關鍵科學問題。采用在有序微納界面上模擬內源電信號刺激的方法，構建基于有序微納
界面與生物電信號的定向誘導組織修復與再生的模型，在分子、細胞、組織等水平闡明微
納界面誘導和內源生物電信號的協同作用機制，為實現皮膚組織的定向修復與再生提供科
學依據。
Endogenous electric signal is fundamentally important for development,
regeneration, and wound healing. This project adopts the recently established
bio-manufacturing and process control methods to control the directed movement
of Gluconacetobacter xylinum through molecular template and microfluidics. In
addition, by constructing engineered phototaxic Gluconacetobacter xylinum,
assembly and arrangement of cellulose fibers is light controlled to achieve a
specific three-dimensional micro-pattern, which will provide highly
sophisticated multi-level tissue engineering scaffolds to induce directed cell
adhesion and aggregation, for regenerative medicine purposes Further, the
micro-patterned materials will be locally modified with conductive materials
to simulate the endogenous electrical signaling to induce and modulate the
distribution, migration, proliferation, and differentiation of fibroblasts or
neurons. t. This project aims to solve the key scientific issue concerning how
to achieve directed induction of skin repair and regeneration through
modulation of endogenous signaling by simulating the endogenous electric
signal on a patterned micro-/nano-surface and also to elucidate the underlying
mechanisms of the additive effect of micro-/nano-surface and biological
electric signal at molecular, cellular, and tissue level. This will provide a
solid scientific basis for directed repair and regeneration of skin tissues.