私密直播全婐app免费大渔直播,国产av成人无码免费视频,男女同房做爰全过程高潮,国产精品自产拍在线观看

當前位置:群英聚首 > 論文著作 > 正文
Taming the wildness of “Trojan-Horse” peptides by charge-guided masking and protease-triggered de-masking for the controlled delivery of anti-tumor agents
來源:時念秋教授個人網站 發布日期:2017-04-14
作者:Shi NQ, Qi XR
關鍵字:“Trojan-Horse” peptides, taming the wildness, charge-guided masking, protease-triggered demasking, controlled delivery
論文來源:期刊
具體來源:ACS Appl Mater Interfaces. 2017. Mar 29;9(12):10519-10529. (SCI, IF=7.145)
發表時間:2017年

Cell-penetrating peptide (CPP), also called “Trojan Horse” peptide, has become a successful approach to deliver various payloads into cells for achieving the intracellular access. However, the "Trojan Horse" peptide is too wild, not just to "Troy", but rather widely distributed in the body. Thus, there is an urgent need to tame the wildness of “Trojan Horse” peptide for targeted delivery of antineoplastic agents to the tumor site. To achieve this goal, we exploit a masked CPP-doxorubicin conjugate platform for targeted delivery of chemotherapeutic drugs using charge-guided masking and protease-triggered de-masking strategies. In this platform, the cell-penetrating function of the positively CPP (D-form

nonaarginine) is abrogated by a negatively shielding peptide (masked CPP), and between them is a cleavable substrate peptide by the protease (MMP-2/9). Protease-triggered

de-masking would occur when the masked CPP reached the MMP-2/9-riched tumor. The

CPP-doxorubicin conjugate (CPP-Dox) and the masked CPP-Dox conjugate (mCPP-Dox)

were used as models for the evaluation of masking and de-masking processes. It was found that exogenous MMP-2/9 could effectively trigger the reversion of CPP-cargo in this conjugate and this trigger adhered to Michaelis-Menten kinetics profile. This conjugate was sensitive to the trigger of endogenous MMP-2/9 and could induce enhanced cytotoxicity towards MMP-2/9-rich tumor cells. In vivo antitumor efficacy revealed that this masked conjugate had considerable antitumor activity and could inhibit the tumor growth at a higher level relative to CPP-cargo. Low toxicity in vivo showed the noticeably decreased

wildness of this conjugate towards normal tissues and more controllable entry of anti-tumor agents into “Troy”. Based on analyses in vitro and in vivo, this mCPP-cargo conjugate

delivery system held an improved selectivity towards MMP-2/9-rich tumors and would

be a promising strategy for tumor-targeted treatment.

Copyright © 2005 Polymer.cn All rights reserved
中國聚合物網 版權所有
經營性網站備案信息

京公網安備11010502032929號

工商備案公示信息

京ICP證050801號

京ICP備12003651號

主站蜘蛛池模板: 通城县| 乐昌市| 乡宁县| 龙泉市| 资源县| 大悟县| 页游| 博罗县| 安庆市| 云林县| 洛隆县| 通山县| 马尔康县| 郯城县| 永靖县| 临湘市| 竹山县| 敖汉旗| 瑞金市| 深州市| 防城港市| 随州市| 新宾| 满城县| 抚州市| 全椒县| 江安县| 张北县| 灵寿县| 太原市| 兴义市| 兴城市| 诏安县| 新宾| 赤城县| 吉木萨尔县| 博野县| 曲靖市| 成安县| 南宁市| 濉溪县|