作者：Yongxiang Hong, Peisheng Zhang,* Hong Wang, Maolin Yua, Yong Gao,* Jian Chen*
關(guān)鍵字：Hydrogen sulfide AIE, Photoswitch, FRET, Nanoprobe
論文來源：期刊
具體來源：Sensors and Actuators B: Chemical, 2018, 272: 340–347.
發(fā)表時(shí)間：2018年
Current lysosomal H2S-responsive fluorescent probes usually possess notorious aggregation caused quenching (ACQ) phenomenon and suffer from the inevitable background fluorescence signals. To circumvent this problem, we here combined H2S recognizing aggregation-induced emission (AIE) group with photochromic spiropyran moiety to rational design novel photoswitchable AIE nanoprobe (DNBS-DCM-SP) for lysosomal H2S detection with high spatiotemporal resolution. In the presence of H2S, the electron-withdrawing DNBS moiety will deviate from DNBS-DCM-SP via a H2S-induced OeS bond cleavage, induced a sensitive turn-on fluorescence response at about 592 nm. The prepared nanoprobe displays good sensitivity (～5.0 nM), and high specificity (especial against other thiols). In additon, the fluorescence of the H2S-activated AIE nanoprobe (DCM-SP) can be reversibly switched by selective fluorescence resonance energy transfer (FRET) from the AIE fluorophore (DCM) to
ring-opened state of spiropyran via alternating UV/visible light irradiation. Moreover, the feasibility of the prepared photoswitchable AIE nanoprobe is also demonstrated by mapping the production of endogenous H2S in living cells as well as reversible dual-color fluorescence imaging.