私密直播全婐app免费大渔直播,国产av成人无码免费视频,男女同房做爰全过程高潮,国产精品自产拍在线观看

當前位置:群英聚首 > 論文著作 > 正文
Design, optimization and evaluation of specific affinity adsorbent for oligopeptides
來源:袁直教授個人網站 發布日期:2011-07-15
作者:Yitao Qiao, Pinglin Li, Yingchao Chen, Jing Feng, Jun Wang, Wei Wang, Yi Ma, Pingchuan Sun, Zhi Yuan
關鍵字:Affinity adsorbent, Specific adsorption, Middle molecular peptides, Hydrophobic inclusion, Cooperation
論文來源:期刊
發表時間:2010年

A major challenge in the development of affinity adsorbents is the design of specific adsorbents for target molecules. In this paper, a two-step strategy was used to design a specific adsorbent for oligopeptides. Based on the structural characteristics of target peptide DFLAE (DE5), the affinity ligand CDenHis bearing
hydrophobic inclusion and electrostatic interaction sites was prepared by grafting histidine onto -cyclodextrin (CD) using ethylenediamine; ligands with single hydrophobic inclusion or electrostatic interaction sites (CDen and HisOMe) were used as reference ligands. Results indicated that the binding affinity (Ka) of CDenHis with DE5 was 6.23×104M−1, 23- and 61-fold higher than that of CDen and HisOMe, respectively. Computer simulations were used to further optimize the steric configuration of CDenHis. It was found that the optimized ligand CDdnHis exhibited a much improved binding affinity for DE5 (Ka = 1.02×105M−1). Moreover, the corresponding adsorbent A-CDdnHis not only showed much better adsorption ability compared with A-CDenHis, but also excellent adsorption specificity for
DE5-containing peptides. Kinetic analysis and adsorption mechanism studies suggested that the configuration matching of CDdnHis with DE5 and the cooperation of multiple interactions led to the fast and selective adsorption of DE5-containing peptides to A-CDdnHis.

Copyright © 2005 Polymer.cn All rights reserved
中國聚合物網 版權所有
經營性網站備案信息

京公網安備11010502032929號

工商備案公示信息

京ICP證050801號

京ICP備12003651號

主站蜘蛛池模板: 蓬溪县| 房山区| 彭山县| 菏泽市| 巫山县| 霍山县| 新乡县| 万山特区| 阳原县| 甘德县| 柘城县| 德阳市| 曲麻莱县| 基隆市| 大连市| 彭阳县| 咸阳市| 焉耆| 江达县| 康定县| 迭部县| 堆龙德庆县| 丰镇市| 徐州市| 芦溪县| 枞阳县| 政和县| 枣阳市| 延津县| 克拉玛依市| 房产| 育儿| 安岳县| 湛江市| 宣城市| 安达市| 甘泉县| 遂溪县| 右玉县| 泰兴市| 洪泽县|