私密直播全婐app免费大渔直播,国产av成人无码免费视频,男女同房做爰全过程高潮,国产精品自产拍在线观看

當前位置:群英聚首 > 論文著作 > 正文
Covalent Immobilization of Proteins on 3D Poly (acrylic acid) Brushes: Mechanism Study and a More Effective and Controllable Process
來源:陳凱敏副教授個人網(wǎng)站 發(fā)布日期:2014-10-07
作者:Qu, Zhenyuan; Chen, Kaimin; Gu, Hongchen; Xu, Hong
關鍵字:無
論文來源:期刊
具體來源:Bioconjugate Chemistry, 2014, 25(2), 370-378
發(fā)表時間:2014年
Polymeric brushes have emerged as a novel 3D material platform that provides great amounts of binding sites for biomolecules. This paper investigates the covalent immobilization mechanism of protein by spherical poly(acrylic acid) brushes (SPAABs) in the widely adopted N-hydroxysuccinimide / N-(3-dimethyl- aminopropyl)-N’-ethylcarbodiimide hydrochloride (NHS/EDC) process. It was discovered that electrostatic interaction plays a crucial role in the covalent immobilization of protein. Due to the existence of 3D architecture and “Donnan effect”, SPAABs exhibit quite different immobilization kinetics in comparison with conventional 2D materials. Under conditions favorable to electrostatic interaction, the effect of “electrostatic interaction induced covalent binding” was observed as a result of competitive immobilization by physical adsorption and chemical binding. On the basis of the mechanism study, a new “chemical conjugation after electrostatic entrapment” (CCEE) method was developed which set the chemical and physical immobilization process apart. A more effective and well-defined covalent immobilization was achieved. And the binding capacity can be tuned in a wide range (0~4.2 mg protein /mg SPAABs) with a high level of control.
Copyright © 2005 Polymer.cn All rights reserved
中國聚合物網(wǎng) 版權所有
經(jīng)營性網(wǎng)站備案信息

京公網(wǎng)安備11010502032929號

工商備案公示信息

京ICP證050801號

京ICP備12003651號

主站蜘蛛池模板: 绥棱县| 宜宾县| 惠来县| 营山县| 乐平市| 玛纳斯县| 新龙县| 繁峙县| 雷山县| 崇信县| 柏乡县| 林口县| 青阳县| 博爱县| 安乡县| 连江县| 长泰县| 奉新县| 泰安市| 古田县| 海城市| 栾城县| 青龙| 萨迦县| 石泉县| 独山县| 防城港市| 陆河县| 沂南县| 平乡县| 西畴县| 廉江市| 海宁市| 辛集市| 庄浪县| 嘉祥县| 杭锦旗| 金塔县| 巢湖市| 中西区| 武功县|