私密直播全婐app免费大渔直播,国产av成人无码免费视频,男女同房做爰全过程高潮,国产精品自产拍在线观看

當前位置:群英聚首 > 論文著作 > 正文
ChemEurJ--Cyclodextrin-derived mimic of glutathione peroxidase exhibiting enzymatic specificity and high catalytic efficiency
來源:董澤元教授個人網(wǎng)站 發(fā)布日期:2014-10-20
作者:Dong, ZY; Huang, X; Mao, SZ; et al.
關(guān)鍵字:Cyclodextrin, Glutathione Peroxidase, Artificial Enzymes
論文來源:期刊
發(fā)表時間:2006年
To elucidate the relationships between molecular recognition and catalytic ability, we chose three assay systems using three different thiol substrates, glutathione (GSH), 3-carboxyl-4-nitrobenzenethiol (CNBSH), and 4-nitrobenzenethiol (NBSH), to investigate the glutathione peroxidase (GPx) activities of 2,2′-ditellurobis(2-deoxy-β-cyclodextrin) (2-TeCD) in the presence of a variety of structurally distinct hydroperoxides (ROOH), H2O2, tert-butyl peroxide (tBuOOH), and cumene peroxide (CuOOH), as the oxidative reagent. A comparative study of the three assay systems revealed that the cyclodextrin moiety of the GPx mimic 2-TeCD endows the molecule with selectivity for ROOH and thiol substrates, and hydrophobic interactions are the most important driving forces in 2-TeCD complexation. Furthermore, in the novel NBSH assay system, 2-TeCD can catalyze the reduction of ROOH about 3.4×105 times more efficiently than diphenyl diselenide (PhSeSePh), and its second-order rate constants for thiol are similar to some of those of native GPx. This comparative study confirms that efficient binding of the substrate is essential for the catalytic ability of the GPx mimic, and that NBSH is the preferred thiol substrate of 2-TeCD among the chosen thiol substrates. Importantly, the proposed mode of action of 2-TeCD imitates the role played by several possible noncovalent interactions between enzymes and substrates in influencing catalysis and binding.
Copyright © 2005 Polymer.cn All rights reserved
中國聚合物網(wǎng) 版權(quán)所有
經(jīng)營性網(wǎng)站備案信息

京公網(wǎng)安備11010502032929號

工商備案公示信息

京ICP證050801號

京ICP備12003651號

主站蜘蛛池模板: 太白县| 东阳市| 板桥市| 那曲县| 绩溪县| 汉源县| 永嘉县| 赤城县| 邳州市| 抚州市| 遂溪县| 白朗县| 特克斯县| 磐石市| 宜川县| 苍南县| 灌南县| 鲁山县| 泸溪县| 仁化县| 清远市| 龙州县| 平果县| 汉沽区| 平山县| 安国市| 晋州市| 庄浪县| 普安县| 徐闻县| 嘉鱼县| 万年县| 杭锦后旗| 库伦旗| 陵川县| 榆社县| 博湖县| 蒲城县| 汝州市| 葫芦岛市| 孟连|