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50. Novel wound dressing based on nanofibrous PHBV/keratin mats
來源:袁江教授個人網(wǎng)站 發(fā)布日期:2015-09-23
作者:Jiang Yuan, Jia Geng, Zhicai Xing, Kyoung-Jin Shim, In-Suk Han, Jung-Chul Kim, Jian Shen, Inn-Kyu Ka
關(guān)鍵字:Keratin for wound dressing
論文來源:期刊
具體來源:Journal of Tissue Engineering and Regenerative Medicine 9(9):1027-1035, 2015
發(fā)表時間:2015年
Keratin is an important protein used for wound healing and tissue recovery. In this study, keratin was ?rst extracted from raw materials and chemically modi?ed to obtain stable keratin (m-keratin). The raw and m-keratin were examined by Raman spectroscopy. The molecular weight of the m-keratin was analysed by SDS–PAGE. Them-keratinwas then blendedwith poly(hydroxybutylate-co-hydroxyvalerate)(PHBV) and electrospun to afford nano?brous mats. These mats were characterized by ?eld emission scanning electronmicroscopy (FE-SEM), electron spectroscopy for chemical analysis (ESCA) and atomic forcemicroscopy (AFM). Fromthe 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) data, it was found that introduction of keratin enhanced cell proliferation. From wound-healing test and histological examination results, itwas shown that the compositemats acceleratedwound recovery remarkably as compared to the PHBV control. It was concluded that PHBV–keratin may be a good candidate as a wound dressing.
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