私密直播全婐app免费大渔直播,国产av成人无码免费视频,男女同房做爰全过程高潮,国产精品自产拍在线观看

相關鏈接
聯系方式
  • 通信地址:長春市前進大街2699號
  • 郵編:130012
  • 電話:0431-85168283
  • 傳真:
  • Email:songwenlong@jlu.edu.cn
當前位置:> 首頁 > 論文著作 > 正文
Soft Matter, 2011, 7, 10803-10811, Role of superhydrophobicity in the biological activity of fibronectin at the cell–material interface
作者:J. Ballester-Beltrán, P. Rico, D. Moratal, Wenlong Song, J. F. Mano, M. Salmerón-Sánchez
關鍵字:superhydrophobicity, cell–material interface, Fibronectin
論文來源:期刊
發表時間:2015年

Protein adsorption and cellular behavior depend strongly on the wettability of substrates. Such studies are scarce for surfaces exhibiting extreme values of contact angles. Fibronectin (FN)adsorption and adhesion of MC3T3-E1 cells were investigated on superhydrophobic polystyrene (SH-PS) surfaces and compared with the corresponding smooth polystyrene (PS) substrate and the control glass. The FN surface density was lower on the SH-PS than on PS, and the adsorbed protein showed altered conformation of cell adhesion domains, as obtained by ELISA with monoclonal antibodies. Cell adhesion occurred on the SH-PS without the formation of mature focal adhesions, as assessed by immunofluorescence for vinculin, talin and paxillin. Correspondingly, the development of the actin cytoskeleton was delayed and without the presence of defined F-actin fibers. FAK phosphorylation was reduced on SH-PS, as compared with PS and the control glass. Also, cell contractility was diminished on the SH-PS as revealed by phosphorylation of myosin light chain (pMLC). Likewise, FN reorganization and secretion were impaired on the superhydrophobic surfaces. Cell proliferation was significantly lower in SH-PS as compared with PS up to 21 days of culture.



Graphical abstract: Role of superhydrophobicity in the biological activity of fibronectin at the cell–material interface
主站蜘蛛池模板: 红安县| 璧山县| 维西| 华阴市| 巩义市| 龙岩市| 清苑县| 松桃| 长顺县| 双柏县| 高密市| 霸州市| 黔东| 富阳市| 靖远县| 霸州市| 宁远县| 扎兰屯市| 庆阳市| 嘉定区| 小金县| 扎兰屯市| 宁武县| 临沭县| 凉山| 绥化市| 威海市| 旺苍县| 砚山县| 昌平区| 陆良县| 贡山| 马公市| 岑巩县| 禹州市| 唐河县| 南漳县| 织金县| 普兰店市| 英吉沙县| 南岸区|