私密直播全婐app免费大渔直播,国产av成人无码免费视频,男女同房做爰全过程高潮,国产精品自产拍在线观看

相關(guān)鏈接
聯(lián)系方式
  • 通信地址:安徽省合肥市經(jīng)開區(qū)九龍路111號安徽大學生命科學學院
  • 郵編:230601
  • 電話:0551-63861493
  • 傳真:
  • Email:tangrp99@iccas.ac.cn
當前位置:> 首頁 > 論文著作 > 正文
Well-defined Block Copolymers for Gene Delivery to Dendritic Cells: Probing the Effect of Polycation Chain-length
作者:tang rp, et al
關(guān)鍵字:Gene Delivery
論文來源:期刊
具體來源:Journal of Controlled Release
發(fā)表時間:2010年
The development of safe and efficient polymer carriers for DNA vaccine delivery requires mechanistic understanding of structure–function relationship of the polymer carriers and their interaction with antigenpresenting cells. Here we have synthesized a series of diblock copolymers with well-defined chain-length using atom transfer radical polymerization and characterized the influence of polycation chain-length on the
physico-chemical properties of the polymer/DNA complexes as well as the interaction with dendritic cells. The copolymers consist of a hydrophilic poly(ethylene glycol) block and a cationic poly(aminoethyl methacrylate) (PAEM) block. The average degree of polymerization (DP) of the PAEM block was varied among 19, 39, and 75, with nearly uniform distribution. With increasing PAEM chain-length, polyplexes formed by the diblock copolymers and plasmid DNA had smaller average particle size and showed higher stability against electrostatic destabilization by salt and heparin. The polymers were not toxic to mouse dendritic cells (DCs) and only displayed chain-length-dependent toxicity at a high concentration (1 mg/mL). In vitro gene transfection efficiency and polyplex uptake in DCs were also found to correlate with chainlength
of the PAEM block with the longer polymer chain favoring transfection and cellular uptake. The polyplexes induced a modest up-regulation of surface markers for DC maturation that was not significantly dependent on PAEM chain-length. Finally, the polyplex prepared from the longest PAEM block (DP of 75) achieved an average of 20% enhancement over non-condensed anionic dextran in terms of uptake by DCs in
the draining lymph nodes 24 h after subcutaneous injection into mice. Insights gained from studying such structurally well-defined polymer carriers and their interaction with dendritic cells may contribute to improved design of practically useful DNA vaccine delivery systems.
© 2009
主站蜘蛛池模板: 明星| 集安市| 和林格尔县| 厦门市| 扶风县| 嘉定区| 靖州| 黄骅市| 县级市| 德钦县| 大余县| 伊通| 田阳县| 抚顺市| 汉阴县| 曲松县| 枣阳市| 伊通| 奉新县| 广安市| 武乡县| 泰宁县| 小金县| 屏东市| 宁城县| 洪雅县| 定边县| 苏州市| 多伦县| 建昌县| 寿光市| 常宁市| 驻马店市| 白银市| 潜山县| 南昌市| 毕节市| 定日县| 德江县| 色达县| 九龙坡区|