具有調控細胞黏附與生長的細菌纖維素復合材料的微納米構筑(Micro-nano-fabrication of bacterial cellulose composites capable of regulating the adherence and growth of Cultured Cells)
Category of Research Project：國家自然科學基金
Number of Research Project：21074041
list of participants ：楊光
beginning and ending dates ：2011年1月-2013年12月
本項目采用已建立的生物制造過程控制的方法，在生物合成過程中經分子模板、微流控和磁調控葡糖醋桿菌的定向運動，控制纖維素纖維的組裝與排列；通過生物活性分子（殼聚糖、膠原、絲素蛋白等）或導電材料（碳納米管、聚苯胺等）的復合改性，同時對纖維素纖維的表面官能團羥基進行修飾與活化，導入多重氫鍵和靜電力等非共價鍵，設計和調控材料與細胞間的相互作用力。通過體系中分子結構、納米結構和微米結構的多尺度效應，研究在組裝過程中的納米尺度效應和界面效應，闡明多組分精細結構的跨尺度形成機制。通過對成纖維細胞、神經元細胞、內皮細胞的黏附、生長、遷移等行為的生物學評價，揭示構筑條件對材料結構與性能的影響；闡明影響生物材料與細胞間相互作用的化學結構與物理因素，并構筑出可調控細胞黏附、生長的新型生物高分子復合材料，為運用于合理設計具有良好生物相容性的細胞培養器和人工器官提供重要科學依據。
This project adopts the established methods of bio-manufacturing and process control, including molecular templating, micro-fluidic technology and magnetically controlled orientation movement of Gluconacetobacter xylinus, to regulate the assembling and arrangement of cellulose fibers in biosynthetic process. The modification of cellulose fibers with bioactive molecules ( chitosan, collagen, silk fibroin, etc.) and/or conducting materials (carbon nanotubes, polyaniline, etc.), as well as the activation of surface hydroxyl groups should be performed to introduce non-covalent bonds like multiple hydrogen bonding and electrostatic attraction, for the design and regulating of interactions between materials and cells. Afterwards, both the nano-scale and interface effects in the assembling process should be investigated, and the scale-span formation mechanism of multi-component and fine structures can be clarified according to the multi-scale effects of molecular structure, micron and nano structures in the cellulose fiber composite system. Finally, by the biological assessment of the adhesion, growth and migration behaviors of fibroblasts, endothelial cells and nerve cells, etc. we attempt to disclose the influence of construction conditions on the material structure and performances, and to clarify the chemical and physical factors affecting the interactions between biomaterial and cells. These studies will help to build up novel biopolymer composites which can regulate the adherence and growth of cells, and to provide an important scientific basis for rational design of cell culture devices and artificial organs with good biocompatibility.
Key words: Bacterial cellulose; fibroblast; endothelial cell; nerve cells; cell adhesion