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(RSC Adv.) Thiol-Ene Photoimmobilization of Chymotrypsin on Polysiloxane Gels for Enzymatic Peptide Synthesis
作者:Wang, M.; Xing, J.; Sun, Y.; Guo, L.; Lin, B.; Yang, H.*
關鍵字:enzyme, polysiloxane
論文來源:期刊
具體來源:RSC Advances, 2018, 8, 11843-11849.
發(fā)表時間:2018年

Chemically

incorporation of enzymes onto polymeric materials has recently attracted

intense scientific attention. Cross-linked polysiloxane gels as a typical

super-hydrophobic support, is a good candidate for supporting enzymes in

low-water organic medium to efficiently catalyze peptide synthesis because the

hydrophobic polysiloxane matrice can prevent water from attacking the

acyl-enzyme intermediate, which is beneficial for the shift in equilibrium to

peptide formation. In this work, we develop a facile strategy to photoimmobilize

olefin-functionalized chymotrypsin onto cross-linked polysiloxane gels via UV-initiated thiol-ene click

chemistry. The impacts of water addition amount, heat-treatment and recyclability

of the immobilized chymotrypsin influencing on the peptide synthesis efficiency

are investigated. Compared with the native chymotrypsin,

polysiloxane-immobilized chymotrypsin showed advantageous catalytic activity,

higher thermal stability and superior recyclability.





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