(RSC Adv.) Thiol-Ene Photoimmobilization of Chymotrypsin on Polysiloxane Gels for Enzymatic Peptide Synthesis
作者：Wang, M.; Xing, J.; Sun, Y.; Guo, L.; Lin, B.; Yang, H.*
關鍵字：enzyme, polysiloxane
論文來源：期刊
具體來源：RSC Advances, 2018, 8, 11843-11849.
發表時間：2018年
Chemically
incorporation of enzymes onto polymeric materials has recently attracted
intense scientific attention. Cross-linked polysiloxane gels as a typical
super-hydrophobic support, is a good candidate for supporting enzymes in
low-water organic medium to efficiently catalyze peptide synthesis because the
hydrophobic polysiloxane matrice can prevent water from attacking the
acyl-enzyme intermediate, which is beneficial for the shift in equilibrium to
peptide formation. In this work, we develop a facile strategy to photoimmobilize
olefin-functionalized chymotrypsin onto cross-linked polysiloxane gels via UV-initiated thiol-ene click
chemistry. The impacts of water addition amount, heat-treatment and recyclability
of the immobilized chymotrypsin influencing on the peptide synthesis efficiency
are investigated. Compared with the native chymotrypsin,
polysiloxane-immobilized chymotrypsin showed advantageous catalytic activity,
higher thermal stability and superior recyclability.