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[Macromol. Biosci.] Direct Biomolecules Binding on Nonfouling Surface via Newly Discovered Supramolecular Self-assembly under Physiological Condition
作者:Peng Yang
關(guān)鍵字:Protein Self-assembling
論文來源:期刊
具體來源:Macromolecular Bioscience
發(fā)表時間:2012年
When lysozyme is dissolved in a neutral HEPES buffer solution (pH?=?7.4) with 0.001-0.050?M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue" that binds strongly and quickly onto non-fouling coatings. This binding is highly selective towards lysozyme, and excludes synthetic, chemical/physical activation/deactivation (blocking) steps. By using biotinylated lysozyme, such a phase transition quickly creates a perfect biotinylated surface on non-fouling surfaces for avidin binding, showing great potential for the development of low-cost and practical biochips.
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