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It is significant to develop probes for rapid, selective, and sensitive detection of the highly toxic hydrazine in both environmental and biological science. In this work, under mild condition, through the electrostatic attraction between negatively charged CDs and positively charged hemicyanine molecules, a novel ratiometric fluorescence probe containing CDs and a hemicyanine derivative was fabricated for reliable, selective, and sensitive sensing of hydrazine. This nanohybrid system possesses dual emission peaks at 550 and 610 nm under a single excitation wavelength of 530 nm. The addition of hydrazine to CDs-hemicyanine nanohybrid solution results in complete fluorescence quenching of the hemicyanine derivative, while the orange fluorescence of CDs keeps constant. Furthermore, the CDs-hemicyanine nanohybrid system shows high selectivity toward hydrazine over other various species including some nucleophilic species, metal ions and anions. The limit of quantification (LOQ) value was 0–1 mM and the detection limit could be as low as 8.0 μM.