Fabrication of Metal Ion–BSA Chelation-Targeted Surface-Imprinted Particles for Protein Separation
作者：Ting Wang, Yanting Lyu*, Zuoting Yang, Haichuan Cheng, Qiuyu Zhang, and Baoliang Zhang*
關鍵字：Adsorption,Genetics,Inorganic compounds,Layers,Materials
論文來源：期刊
具體來源：Industrial & Engineering Chemistry Research
發表時間：2023年
The development of high-performance protein-imprinted materials for adsorption and separation to obtain high-purity, high-quality biomolecules is still one of the most important techniques to promote the rapid development of the protein drug industry. In this paper, a novel highly specific bovine serum albumin (BSA) cobalt–molybdenum double-hydroxides surface-imprinted composite particles (Co–Mo LDHs@MIPs) were constructed by the surface imprinting technique combined with metal chelation. ZIF-67 was etched by molybdate ions to obtain unique MOF-derived bimetallic hydroxide particles (Co–Mo LDHs), which were used as the carrier. The rough outer surface and bimetallic ion coordination of Co–Mo LDHs enable anchoring more template proteins. The adsorption capacity of Co–Mo LDHs@MIPs was up to 423.37 mg/g for BSA in 60 min with an imprinting factor of 2.65. In addition, Co–Mo LDHs@MIPs can selectively recognize and separate BSA from mixed proteins and fetal bovine serum and exhibit high reusability stability.