Ratiometric imaging of lysosomal hypochlorous acid enabled by FRET-based polymer dots
作者：Hong Wang, Peisheng Zhang,* Yongxiang Hong, Bin Zhao, Pinggui Yi and Jian Chen*
關鍵字：HClO, polymer dots, Lysosome,
論文來源：期刊
具體來源：Polym. Chem.,2017, 8,5795–5802
發表時間：2017年
The development of an effective method for imaging of lysosomal hypochlorous acid (HClO) is of great significance to better understand the roles of HClO-related diseases. Herein, we report on the fabrication of fluorescence resonance energy transfer (FRET)-based fluorescent polymer dots (FPD) for the ratiometric imaging of lysosomal HClO in living cells via a simple co-precipitation method. This FRET-based construction strategy takes the advantage of flexible design by incorporating target-inert fluorogen (donor) and target-sensitive fluorogen (acceptor) into the nanoparticle to avoid leakage and photobleaching problems. The as-prepared FPD exhibits good water dispersibility, fast response (<30 s), high sensitivity (～5.1 nM), outstanding selectivity, excellent photostability and long-term stability (>15 weeks). Moreover, the resultant FPD with good membrane permeability could mainly locate in the lysosomes and act as a probe for the ratiometric imaging of lysosomal HClO in living HeLa cells.