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The controllable preparation of porous PLGA microspheres by the oil/water emulsion method and its application in 3D culture of ovarian cancer cells
作者:Tianzhu Zhang*, Qiying Zhang, Junsong Chen, Kun Fang, Jun Dou, Ning Gu*
關(guān)鍵字:PLGA Smooth microsphere,Porosity,3D culture,Ovarian cancer cells
論文來(lái)源:期刊
具體來(lái)源:Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2014, 452: 115–124
發(fā)表時(shí)間:2014年
In this study, we prepared surface-smooth and porous poly(lactic-co-glycolic acid) (PLGA) microsphereswith different diameters, different sizes of pores and different densities of pores using a simple oil/wateremulsion method and varying the preparation conditions, including the molecular weight of PLGA, thecomponents of the PLGA and the addition of porogen. The surface-smooth nonporous and porous PLGAmicrospheres were also further modified with collagen I. The characteristics of these PLGA microspheres,including original and collagen I-coated PLGA microspheres, were evaluated in a three-dimensional (3D)culture of ovarian cancer HO8910 cells. The HO8910 cells can growth better on original porous andcollagen I-coated PLGA microspheres, and express remarkably E-cadherin. These results indicate thatporous PLGA microspheres and collagen-coated PLGA microspheres are promising candidates as ovariancancer cell culture microcarriers for pathological study and high-throughput antitumor drug screening.
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