私密直播全婐app免费大渔直播,国产av成人无码免费视频,男女同房做爰全过程高潮,国产精品自产拍在线观看

相關鏈接
聯系方式
  • 通信地址:南開區衛津路94號
  • 郵編:300071
  • 電話:86-22-23501657
  • 傳真:86-22-23503510
  • Email:yongjunzhang@nankai.edu.cn
當前位置:> 首頁 > 論文著作 > 正文
Investigation and intervention of autophagy to guide cancer treatment with nanogels
作者:Xudong Zhang,? Xin Liang,? Jianjun Gu,? Danfeng Chang, Jinxie Zhang, Zhaowei Chen, Yanqi Ye, Chao Wa
關鍵字:microgel
論文來源:期刊
具體來源:Nanoscale, 2017, 9(1), 150-163
發表時間:2017年

Cancer cells use autophagy to resist poor survival environmental conditions such as low PH, poor nutrients as well as chemical therapy. Nanogels have been used as efficient chemical drug carriers for cancer treatment. However, the effect of nanogels on autophagy is still unknown. Here, we used Rab proteins as the marker of multiple trafficking vesicles in endocytosis and LC3 as the marker of autophagy to investigate the intracellular trafficking network of Rhodamine B (Rho)-labeled nanogels. The nanogels were internalized by the cells through multiple protein dependent endocytosis and micropinocytosis. After inception by the cells, the nanogels were transported into multiple Rab positive vesicles including early endosomes (EEs), late endosomes (LEs), recycling endosomes (REs) and lipid droplets. Finally, these Rab positive vesicles were transported to lysosome. In addition, GLUT4 exocytosis vesicles could transport the nanogels out of the cells. Moreover, nanogels could induce autophagy and be sequestered in autophagosomes. The crosstalk between autophagosomes and Rab positive vesicles were investigated, we found that autophagosomes may receive nanogels through multiple Rab positive vesicles. Co-delivery of autophagy inhibitors such as chloroquine (CQ) and the chemotherapeutic drug doxorubicin (DOX) by nanogels blocked the autophagy induced by DOX greatly decreasing both of the volume and weight of the tumors in mice tumor models. Investigation and intervention of the autophagy pathway could provide a new method to improve the therapeutic effect of anticancer nanogels.



主站蜘蛛池模板: 阳城县| 武冈市| 鹿邑县| 岳阳市| 从化市| 武功县| 汉阴县| 邢台县| 探索| 宝坻区| 玉田县| 雅江县| 新津县| 宁明县| 竹溪县| 寻乌县| 勃利县| 吕梁市| 读书| 比如县| 惠水县| 衡阳市| 三明市| 抚顺县| 布尔津县| 甘孜| 荔波县| 集安市| 西乡县| 定州市| 德阳市| 牙克石市| 龙江县| 贡嘎县| 方山县| 桂东县| 扎兰屯市| 防城港市| 襄垣县| 枝江市| 思南县|