私密直播全婐app免费大渔直播,国产av成人无码免费视频,男女同房做爰全过程高潮,国产精品自产拍在线观看

當前位置:> 首頁 > 論文著作 > 正文
Reducible poly(amido ethylenimine) directed to enhance RNA interference

Designing synthetic macromolecular vehicles with high transfection efficiency and low cytotoxicity has been a major interest in the development of non-viral gene carriers. A reducible poly(amido ethylenimine) (SS-PAEI) synthesized by addition copolymerization of triethylenetetramine and cystamine bis-acrylamide (poly(TETA/CBA)) was used as a carrier for small interference RNA (siRNA). Poly(TETA/CBA) could efficiently condense siRNA to form stable complexes under physiological conditions and perform complete release of siRNA in a reductive environment. When formulated with VEGF-directed siRNA, poly(TETA/CBA) demonstrated significantly higher suppression of VEGF than linear-polyethylenimine (PEI) (L-PEI, 25 kDa) in human prostate cancer cells (PC-3). After 5 h of transfection, substantial dissociation and intracellular distribution of siRNA was observed in the poly(TETA/CBA) formulation, but not in the L-PEI formulation. The triggered release of siRNA by reductive degradation of poly(TETA/CBA) in the cytoplasm may affect the RNAi activity by increasing cytoplasmic availability of siRNA. These results suggest that the rational design of non-viral carriers should involve considerations for intracellular dissociation and trafficking of a nucleic acid drug to maximize its effect, in conjunction with formation of stable complexes under physiological conditions.

主站蜘蛛池模板: 高尔夫| 石棉县| 深水埗区| 桃江县| 丰镇市| 房产| 汕尾市| 江陵县| 平昌县| 扎兰屯市| 镇宁| 海淀区| 东阳市| 比如县| 永吉县| 萍乡市| 荣成市| 海晏县| 无极县| 天气| 黔东| 南平市| 临沂市| 依兰县| 镇赉县| 肃北| 衡阳市| 滨海县| 乌兰县| 南召县| 云和县| 津南区| 澄迈县| 吉林市| 习水县| 子长县| 瑞昌市| 沙田区| 鄂州市| 左权县| 察雅县|