私密直播全婐app免费大渔直播,国产av成人无码免费视频,男女同房做爰全过程高潮,国产精品自产拍在线观看

當前位置:> 首頁 > 論文著作 > 正文
A versatile family of degradable non-viral gene carriers based on hyperbranched poly(ester amine)s

A variety of degradable hyperbranched poly(ester amine)s containing primary, secondary and tertiary amino groups, were synthesized and evaluated as non-viral gene carriers. The polymers were obtained in high yields through a Michael-type conjugate addition of diacrylate monomers with trifunctional amine monomers. Analysis of degradation products using liquid chromatography-mass spectroscopy (LC-MS) demonstrated that all poly(ester amine)s had a hyperbranched structure with a degree of branching of approximately 0.30. These poly(ester amine)s were readily water-soluble and degradable under physiological conditions (pH 7.4, 37 degrees C), in which more than 10% ester bonds were hydrolyzed within 4 h. Moreover, these hyperbranched poly(ester amine)s showed high buffering capacities between pH 5.1 and 7.4. Three out of nine synthesized polymers, i.e. p(HDDA-AEP), p(HDDA-AMP), and p(BDDA-AMP), were shown to effectively condense plasmid DNA into small-sized (similar to 94-135 nm) and positively charged complexes. Polymer/DNA complexes ('polyplexes') based on these three polymers, and larger complexes of p(BDDA-AEP) (similar to 497 nm) were able to transfect COS-7 cells in vitro. Importantly, the transfection activity of polyplexes was preserved in the presence of serum proteins. The highest transfection level was observed for p(HDDA-AEP) polyplex which had a transfection efficiency higher-than or comparable to that polyplexes of polyethylenimine (PEI) and poly(2-(dimethylamino)ethyl methacrylate) (pDMAEMA). Furthermore, these poly(ester amine)s revealed no or low cytotoxicity. These results demonstrated that hyperbranched poly(ester amine)s can be applied as safe and efficient gene delivery polymers.

主站蜘蛛池模板: 富锦市| 连州市| 明光市| 洪江市| 兴海县| 内丘县| 常熟市| 益阳市| 射阳县| 石城县| 金乡县| 洱源县| 阿瓦提县| 科尔| 青铜峡市| 抚远县| 巍山| 陕西省| 旺苍县| 诸城市| 阿拉善右旗| 楚雄市| 西林县| 吉安县| 化德县| 积石山| 三门峡市| 景德镇市| 孟津县| 日喀则市| 蓬溪县| 河津市| 杂多县| 当涂县| 安新县| 平罗县| 桃江县| 玛沁县| 萝北县| 华宁县| 绥芬河市|