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 | 濉戞枡 | 姍¤兌 | 綰ょ淮 | 娑傛枡涓庢補(bǔ)澧?/a> | 鑳剁矘鍓?/a> | 澶嶅悎鏉愭枡 | 鐭沖寲 | 鍖栧伐 | 浠櫒浠〃 | 鏂頒駭涓?/a> | 涓撻 | 榪斿洖棣栭〉 |
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| 濉戞枡 | 姍¤兌 | 綰ょ淮 | 娑傛枡涓庢補(bǔ)澧?/a> | 鑳剁矘鍓?/a> | 澶嶅悎鏉愭枡 | 鐭沖寲 | 鍖栧伐 | 浠櫒浠〃 | 鏂頒駭涓?/a> | 涓撻 | 榪斿洖棣栭〉 |
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璇ヨ棰樼粍涓烘彁楂?/span>GEN鐨勭敓鐗╁埄鐢ㄧ巼錛屾彁楂樺叾闈跺悜鎬э紝鍒跺浜嗗惈鏈夎秴姘у寲鐗╂鍖栭叾妯℃嫙鐗╋紙Tempol錛夊叡杞殑 尾-鐜硦綺懼拰 4-錛堢緹鐢插熀錛夎嫰鍩虹〖閰擱鍝唶閰慨楗扮殑GEN鐨?/span>ROS鍝嶅簲鍨嬬撼綾蟲潗鏂欙紙GEN-NP2錛夈傝鏉愭枡鍙互琚偁涓婄毊緇嗚優(yōu)鎽勫彇騫跺湪鐐庣棁閮ㄤ綅ROS鍒烘縺涓嬮珮鏁堟竻闄ゅ悇縐嶆椿鎬ф哀鍒嗗瓙錛堝浘1錛夈傚彟澶?/span>GEN-NP2鍙互淇濇姢 GEN 鍏嶅彈鑳冭偁閬撲腑娑堝寲閰剁殑蹇熶唬璋紝浠庤屾湁鏁堝湴灝?/span>GEN 闈跺悜閫掗佽嚦鐐庣棁閮ㄤ綅錛堝浘2錛夈備粠鏈哄埗涓婅錛?/span>GEN-NP2閫氳繃澧炲姞闆屾縺绱犲彈浣?/span>尾錛?/span>ER尾錛夌殑琛ㄨ揪錛岄檷浣庝績鐐庝粙璐ㄧ殑琛ㄨ揪錛屽噺寮辯値鐥囬儴浣嶇値鐥囩粏鑳?yōu)鐨剫垢娑﹀Q屼績榪涜偁涓婄毊緇嗚優(yōu)鐨勮嚜鍣紝鎶戝埗鐐庢х粏鑳?yōu)鍥犲瓙鐨勫垎娉屽Q屽茍璋冭妭鑲犻亾寰敓鐗╃兢鐨勭ǔ鎬侊紝鏈緇堢紦瑙ｈ偁閬撶値鐥囷紙鍥?/span>3錛?/span>4錛夈傛湰鐮旂┒涓?/span> IBD 鐨勭簿紜不鐤楁彁渚涗簡涓縐嶆湁鏁堢殑鏂規(guī)錛屼負(fù)鎻愰珮涓嵂鐨勭敓鐗╁埄鐢ㄥ害錛屾不鐤楀叾瀹冧笌姘у寲搴旀縺銆佺値鐥囩浉鍏崇殑鐤劇梾鎻愪緵浜嗘濊礬銆?/span>
鍥?/span> 2. GEN-NPs 闈跺悜瀹氫綅浜?span>鍏鋒湁鐐?span>鐥囧弽搴?/span>鐨勭粨鑲犮?/span>(A-D) 閫氳繃绱鍙鍏夎氨 (A鍜?/span>C) 鍜?/span>HPLC (B鍜?/span>D) 媯嫻?SGF銆?/span>SIF鍜?/span>SCF 涓?GEN-NPs 鐨勫寲瀛︾ǔ瀹氭с?/span>(E) GEN鍦ㄤ笉鍚屾ā鎷熺紦鍐叉憾娑蹭腑浠?GEN-NPs鐨勯噴鏀炬洸綰褲?/span>(F) FITC鏍囪鐨?GEN-NP1鍦ㄥ仴搴鋒垨緇撹偁鐐庡皬榧犱腑紱諱綋鎴愬儚鐨勭敓鐗╁垎甯冨垎鏋愩?/span>(G) 緇撹偁涓?/span>FITC-GEN-NP1鑽у厜淇″彿鐨勯噺鍖栥?/span>
鍥?/span>3. GEN-NPs 璋冭妭緇嗚優(yōu)涓殑鑷櫖-鐐庣棁灝忎綋淇″彿閫氳礬銆?/span>(A) 浣跨敤铔嬬櫧璐ㄥ嵃榪瑰垎鏋?ASC銆?/span>Caspase1-p20銆?/span>ULK1銆?/span>ATG16L1 鍜?ER尾 鐨勮〃杈炬按騫熾?/span>(B) ER尾浜岃仛鍖栫殑媯嫻嬨?/span>(C) 緇嗚優(yōu)涓殑 EGFP-mCherry-LC3B錛堥粍鑹詫級琛ㄨ揪銆?/span>(D)鑷櫖鐩稿叧鏂戠偣闃蟲?/span>緇嗚優(yōu)瀹氶噺鍒嗘瀽銆?/span>
鍥?/span>4. GEN-NPs 瀵硅偁閬撳井鐢熺墿緹ょ殑璋冭妭浣滅敤銆?/span>(A) 綺究寰敓鐗╃兢緇勬垚鍦ㄥ睘姘村鉤涓婄殑鍙樺寲銆?/span>(B) 涓嶅悓澶勭悊緇勭殑鑲犻亾寰敓鐗╃兢 偽 澶氭牱鎬с?/span>(C) 涓嶅悓澶勭悊緇勪腑寰敓鐗╃兢钀界殑棣欏啘鎸囨暟銆?/span> (D) 浠ｈ〃鎬ф牱鍝佷腑寰敓鐗╃兢緇勬垚鐨?NMDS 鍒嗘瀽銆?/span>
鐩稿叧閾炬帴
https://pubs.acs.org/doi/pdf/10.1021/acsami.1c09215