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Fabricating a cycloolefin polymer immunoassay platform with a dual-function polymer brush via a surface-initiated photoiniferter-mediated polymerization strategy  
Fabricating a cycloolefin polymer immunoassay platform with a dual-function polymer brush via a surface-initiated photoiniferter-mediated polymerization strategy
資料類型: PDF文件
關鍵詞: cycloolefin  polymer  (COP)  surface-initiated  photoiniferter-mediated  polymerization  (SI-PIMP)  
hierarchical  architecture  antibody  immobilization  immunoassay  
資料大小: k
所屬學科: 高分子工程
來源: 來源網絡
簡介:
The development of technologies for abiomedical detection platform is critical to meet the globalchallenges of various disease diagnoses. In this study, an inertcycloolefin polymer (COP) support was modified with two-layerpolymer brushes possessing dual functions, i.e., a lowfouling poly[poly(ethylene glycol) methacrylate] [p-(PEGMA)] bottom layer and a poly(acrylic acid) (PAA)upper layer for antibody loading, via a surface-initiatedphotoiniferter-mediated polymerization strategy for fluorescence-based immunoassay. It was demonstrated through aconfocal laser scanner that, for the as-prepared COP-g-PEG-b-PAA-IgG supports, nonspecific protein adsorption was suppressed,and the resistance to nonspecific protein interference on antigen recognition was significantly improved, relative to the COP-g-PAA-IgG references. This strategy for surface modification of a polymeric platform is also applicable to the fabrication of otherbiosensors.
上傳人: luanshifang
上傳時間: 2014-03-23 5:25:23
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