私密直播全婐app免费大渔直播,国产av成人无码免费视频,男女同房做爰全过程高潮,国产精品自产拍在线观看

搜索:  
Distinct transduction modes of arginine-rich cell-penetrating peptides for cargo delivery into tumor cells  
Distinct transduction modes of arginine-rich cell-penetrating peptides for cargo delivery into tumor cells
資料類型: PDF文件
關(guān)鍵詞: Cell-penetrating  peptides  Endocytosis  Heparan  sulfate  proteoglycans  Macropinocytosis  
Nona-arginine  
資料大小: 1001k
所屬學(xué)科: 功能高分子
來源: 來源網(wǎng)絡(luò)
簡(jiǎn)介:
The application of cell-penetrating peptides (CPPs) for delivering various cargo molecules with biologicalfunctions into cells has gained much attention in recent years. However, the internalizationmechanisms and delivery properties of CPP–cargo remains controversial. In this study, low- and highmolecular-weight cargoes attached to arginine-rich CPPs were employed: the former was the fluoresceinisothiocyanate-labeled nona-arginine (CPP–FITC), and the latter was the fluorescently labeled nonaarginine–avidin complex (CPP–avidin). We measured the intracellular trafficking of CPP–FITC andCPP–avidin in four cancer cell lines in a series of microenvironments altered by the presence or absenceof serum, different temperatures and different incubation times. The results revealed that CPP–cargodelivery exhibited no specificity toward any cell line, but the levels were found to be related to cell type and cargo. Furthermore, their endocytic mechanisms were investigated via incubation with relatedendocytic inhibitors. Two different types of CPP–cargo were required to cross the plasma membraneto bind to cell surface-associated heparan sulfate proteoglycans in a time-dependent manner. CPPs andsmall cargoes attached to CPP may enter cells rapidly via direct translocation in addition to the endocyticroute. Translocation of large components linked to CPP tended to be mediated by macropinocytosis in anenergy-dependent manner with slower rates for larger compounds. In contrast, the clathrin-dependentpathway is not essential to the translocation of either type of CPP–cargo.
上傳人: shinianqiu
上傳時(shí)間: 2017-04-14 12:51:16
下載次數(shù): 0
消耗積分: 0  
立即下載:
1人
1人
1人
友情提示:下載后對(duì)該資源進(jìn)行評(píng)論,即可獎(jiǎng)勵(lì)2分。
報(bào)告錯(cuò)誤:  1.下載無效  2. 資料明顯無價(jià)值  3. 資料重復(fù)存在

相關(guān)評(píng)論 共有0人發(fā)表評(píng)論 更多評(píng)論
你還沒有登錄,無法發(fā)表評(píng)論,請(qǐng)首先 登錄 注冊(cè)
免責(zé)聲明:本站部分資源由網(wǎng)友推薦,來自互聯(lián)網(wǎng),版權(quán)屬于原版權(quán)人,如果不慎侵犯到您的權(quán)利,敬請(qǐng)告知,我們會(huì)在第一時(shí)間撤除。本站中各網(wǎng)友的評(píng)論只代表其個(gè)人觀點(diǎn),不代表本站同意其觀點(diǎn)。
主站蜘蛛池模板: 景泰县| 巴塘县| 安宁市| 青田县| 鄂托克前旗| 蒲城县| 大埔区| 调兵山市| 四川省| 丁青县| 岫岩| 河东区| 周至县| 深水埗区| 南投县| 达拉特旗| 宁国市| 金寨县| 舒城县| 呼伦贝尔市| 汝南县| 黄陵县| 郧西县| 西乌珠穆沁旗| 信丰县| 绥化市| 东明县| 龙海市| 磐安县| 深泽县| 青河县| 金川县| 德安县| 山西省| 望谟县| 应城市| 西乌珠穆沁旗| 绍兴市| 嘉义市| 林州市| 长海县|