私密直播全婐app免费大渔直播,国产av成人无码免费视频,男女同房做爰全过程高潮,国产精品自产拍在线观看

當前位置:群英聚首 > 論文著作 > 正文
Journal of Materials Chemistry 2011: Dendrimer modified magnetic iron oxide nanoparticle/DNA/PEI ternary magnetoplexes: a novel strategy for magnetofection
來源:黃世文教授個人網站 發布日期:2011-11-11
作者:Liu Wen-Ming, Xue Ya-Nan, Peng Na, He Wen-Tao, Zhuo Ren-Xi, Huang Shi-Wen
關鍵字:Gene delivery
論文來源:期刊
具體來源:JOURNAL OF MATERIALS CHEMISTRY 2011, 21, 13306-13315
發表時間:2011年

As a powerful technology to enhance the efficiency of gene delivery, magnetofection has attracted considerable attention in the past decade. In this work, we introduced 6 generation of PAMAM dendrimer modified superparamagnetic nanoparticles (DMSPION-G6) to PEI/DNA polyplexes by a two-step process and enhanced the transfection efficiency of PEI with the help of a magnetic field. We prepared DMSPION-G6/DNA/PEI ternary magnetoplexes by precondensing DMSPION-G6 with DNA at a low mass ratio to yield DMSPION-G6/DNA magnetoplexes with negative surface charge, followed by further coating with branched PEI (25 kDa) via electrostatic interactions. We measured the transfection efficiencies of DMSPION-G6/DNA/PEI ternary magnetoplexes in COS-7, 293T and HeLa cells in the presence or absence of a magnetic field. Compared with PEI/DNA polyplexes, DMSPION-G6/DNA/PEI ternary magnetoplexes exhibited enhanced transfection efficiencies in all the three cell lines when a magnetic field was applied, especially in the presence of 10% FBS. Time-resolved and dose-resolved transfection indicated that high-level transgene expression was achievable with a relatively short incubation time and low DNA dose when magnetofection was employed. Further evidence from Prussian blue staining, quantification of cellular iron concentration and cellular uptake of Cy-3 labelled DNA demonstrated that the magnetic field could quickly gather the magnetoplexes to the surface of target cells and consequently enhanced the uptake of magnetoplexes by the cells. This represents a novel strategy for polycation-based in vitro gene delivery enhanced by a magnetic field.

Copyright © 2005 Polymer.cn All rights reserved
中國聚合物網 版權所有
經營性網站備案信息

京公網安備11010502032929號

工商備案公示信息

京ICP證050801號

京ICP備12003651號

主站蜘蛛池模板: 长顺县| 剑河县| 邳州市| 扶余县| 绥芬河市| 鱼台县| 志丹县| 大化| 灯塔市| 尉犁县| 富蕴县| 银川市| 余庆县| 文昌市| 安丘市| 南城县| 宁远县| 阜南县| 扎兰屯市| 南平市| 莱州市| 盖州市| 贞丰县| 巴东县| 海盐县| 昌黎县| 麻阳| 东乡族自治县| 珲春市| 晋城| 伊川县| 深圳市| 德化县| 广平县| 湖南省| 阿图什市| 新化县| 屯留县| 鄢陵县| 泽州县| 旌德县|